real time rt pcr assay  (Revvity)

Journal: ACS Omega

Article Title: DirectDetect SARS-CoV-2 Direct Real-Time RT-PCR Study Using Patient Samples

doi: 10.1021/acsomega.1c05595

Figure Lengend Snippet: Detection of SARS-CoV-2 by the commonly used manual real-time reverse transcriptase polymerase chain reaction (RT-PCR) procedure vs the DirectDetect procedure. The overall assay times for these procedures from sample collection to result differ from up to 4 h for the conventional method to up to 2 h for DirectDetect. Created with BioRender.com .

Article Snippet: This study presents and analyzes a new COVID-19 diagnostic test called DirectDetect SARS-CoV-2 Direct Real-time RT-PCR (PerkinElmer, Waltham, MA, USA).

Techniques: Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

Journal: ACS Omega

Article Title: DirectDetect SARS-CoV-2 Direct Real-Time RT-PCR Study Using Patient Samples

doi: 10.1021/acsomega.1c05595

Figure Lengend Snippet: DirectDetect SARS-CoV-2 Direct Real-time RT-PCR sample preparation workflow. Created with BioRender.com .

Article Snippet: This study presents and analyzes a new COVID-19 diagnostic test called DirectDetect SARS-CoV-2 Direct Real-time RT-PCR (PerkinElmer, Waltham, MA, USA).

Techniques: Quantitative RT-PCR, Sample Prep

Journal: ACS Omega

Article Title: DirectDetect SARS-CoV-2 Direct Real-Time RT-PCR Study Using Patient Samples

doi: 10.1021/acsomega.1c05595

Figure Lengend Snippet: Comparison of Selected Individual EUAs for Molecular Diagnostic Tests for SARS-CoV-2 to DirectDetect (Not Approved as an EUA Test) That Do Not Use Nucleic Acid Extraction

Article Snippet: This study presents and analyzes a new COVID-19 diagnostic test called DirectDetect SARS-CoV-2 Direct Real-time RT-PCR (PerkinElmer, Waltham, MA, USA).

Techniques: Comparison, Diagnostic Assay, Amplification, Quantitative RT-PCR, Control

Journal: ACS Omega

Article Title: DirectDetect SARS-CoV-2 Direct Real-Time RT-PCR Study Using Patient Samples

doi: 10.1021/acsomega.1c05595

Figure Lengend Snippet: LoD Results for DirectDetect Using the AccuPlex SARS-CoV-2 Reference Material Kit, n = 12 for Each Group

Article Snippet: This study presents and analyzes a new COVID-19 diagnostic test called DirectDetect SARS-CoV-2 Direct Real-time RT-PCR (PerkinElmer, Waltham, MA, USA).

Techniques: Concentration Assay, Control

Journal: ACS Omega

Article Title: DirectDetect SARS-CoV-2 Direct Real-Time RT-PCR Study Using Patient Samples

doi: 10.1021/acsomega.1c05595

Figure Lengend Snippet: Evaluation of Positive Agreement for Overall SARS-CoV-2 Detection Using DirectDetect and Commercial Real-Time RT-PCR Assay

Article Snippet: This study presents and analyzes a new COVID-19 diagnostic test called DirectDetect SARS-CoV-2 Direct Real-time RT-PCR (PerkinElmer, Waltham, MA, USA).

Techniques: Quantitative RT-PCR

Journal: ACS Omega

Article Title: DirectDetect SARS-CoV-2 Direct Real-Time RT-PCR Study Using Patient Samples

doi: 10.1021/acsomega.1c05595

Figure Lengend Snippet: DirectDetect Kit Components and Reagent Volumes per Sample a

Article Snippet: This study presents and analyzes a new COVID-19 diagnostic test called DirectDetect SARS-CoV-2 Direct Real-time RT-PCR (PerkinElmer, Waltham, MA, USA).

Techniques: Reverse Transcription, Positive Control, Recombinant, Virus, Negative Control

Journal: ACS Omega

Article Title: DirectDetect SARS-CoV-2 Direct Real-Time RT-PCR Study Using Patient Samples

doi: 10.1021/acsomega.1c05595

Figure Lengend Snippet: Patient Sample Evaluation for SARS-CoV-2 Using DirectDetect Real-Time RT-PCR Based on the Ct Value of Each Fluorophore Corresponding with a Different Gene during RT-PCR

Article Snippet: This study presents and analyzes a new COVID-19 diagnostic test called DirectDetect SARS-CoV-2 Direct Real-time RT-PCR (PerkinElmer, Waltham, MA, USA).

Techniques: Quantitative RT-PCR

Journal: Frontiers in Microbiology

Article Title: Factors that Influence the Reported Sensitivity of Rapid Antigen Testing for SARS-CoV-2

doi: 10.3389/fmicb.2021.714242

Figure Lengend Snippet: Mechanism of action for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antigen detection through lateral flow assay design. (1) The specimen analyte, containing SARS-CoV-2 antigen suspended in assay buffer, is deposited in the sample well (at 2). (3) The analyte (containing antigen; in green) is absorbed into the sample pad and begins to diffuse across the reaction chamber into the conjugate pad. (4) The analyte comes into proximity of a SARS-CoV-2-specific antigen antibody that is conjugated to a tag (usually consisting of gold, latex, or a fluorophore). (5) The antigen–antibody complex migrates via diffusion across the nitrocellulose membrane. (6) The SARS-CoV-2 antigen–antibody complex comes into proximity of a second SARS-CoV-2 antigen antibody (different epitope) that is covalently bound to the device pad, and an antibody–antigen–antibody complex forms, resulting in the test line. Further diffusion of excess SARS-CoV-2 antibodies (unbound to antigen) results in association of a second covalently bound antibody that is specific for the first SARS-CoV-2 antibody. (7) An antibody–antibody complex forms resulting in the control line.

Article Snippet: 27 (sx/asx) , , United States , 2,339 , 156 , BinaxNOW TM COVID-19 Ag Card test kit , Abbott Diagnostics Inc., United States , PerkinElmer SARS-CoV-2 RT-PCR , PerkinElmer, Inc., United States.

Techniques: Lateral Flow Assay, Diffusion-based Assay

Journal: Frontiers in Microbiology

Article Title: Factors that Influence the Reported Sensitivity of Rapid Antigen Testing for SARS-CoV-2

doi: 10.3389/fmicb.2021.714242

Figure Lengend Snippet: Information characterizing data sources involving SARS-CoV-2 antigen testing for analyses described in this report.

Article Snippet: 27 (sx/asx) , , United States , 2,339 , 156 , BinaxNOW TM COVID-19 Ag Card test kit , Abbott Diagnostics Inc., United States , PerkinElmer SARS-CoV-2 RT-PCR , PerkinElmer, Inc., United States.

Techniques: Diagnostic Assay, Multiplex Assay, Surround Optical-fiber Immunoassay, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction

Journal: Frontiers in Microbiology

Article Title: Factors that Influence the Reported Sensitivity of Rapid Antigen Testing for SARS-CoV-2

doi: 10.3389/fmicb.2021.714242

Figure Lengend Snippet: Forest plots containing calculated sensitivity values for index [severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antigen test] testing compared to reference [SARS-CoV-2 quantitative reverse transcription polymerase chain reaction (RT-qPCR assay)] test. Data are stratified by days from symptom onset.

Article Snippet: 27 (sx/asx) , , United States , 2,339 , 156 , BinaxNOW TM COVID-19 Ag Card test kit , Abbott Diagnostics Inc., United States , PerkinElmer SARS-CoV-2 RT-PCR , PerkinElmer, Inc., United States.

Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Frontiers in Microbiology

Article Title: Factors that Influence the Reported Sensitivity of Rapid Antigen Testing for SARS-CoV-2

doi: 10.3389/fmicb.2021.714242

Figure Lengend Snippet: Forest plots containing calculated sensitivity values for index [severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antigen test] testing compared to reference [SARS-CoV-2 quantitative reverse transcription polymerase chain reaction (RT-qPCR assay)] test. Data are stratified by viral load [genomic copies/ml (cpm)] and by symptomatic or asymptomatic status.

Article Snippet: 27 (sx/asx) , , United States , 2,339 , 156 , BinaxNOW TM COVID-19 Ag Card test kit , Abbott Diagnostics Inc., United States , PerkinElmer SARS-CoV-2 RT-PCR , PerkinElmer, Inc., United States.

Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR

Journal: Frontiers in Microbiology

Article Title: Factors that Influence the Reported Sensitivity of Rapid Antigen Testing for SARS-CoV-2

doi: 10.3389/fmicb.2021.714242

Figure Lengend Snippet: Forest plots containing calculated sensitivity values for index [severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) antigen test and SARS-CoV-2 quantitative reverse transcription polymerase chain reaction (RT-qPCR assay)] testing compared to reference (SARS-CoV-2 viral culture) test.

Article Snippet: 27 (sx/asx) , , United States , 2,339 , 156 , BinaxNOW TM COVID-19 Ag Card test kit , Abbott Diagnostics Inc., United States , PerkinElmer SARS-CoV-2 RT-PCR , PerkinElmer, Inc., United States.

Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR